Role of TLR5 in the Translocation and Dissemination of Commensal Bacteria in the Intestine after Traumatic Hemorrhagic Shock

Figure 1

Effect of TLR5 deficiency on the translocation and dissemination of commensal bacteria. Bioluminescent C. rodentium, visualized using an in vivo imaging system (IVIS), was used to monitor bacterial translocation. (a) The panel of LB agar plates with different tissue homogenates was shown in the top left corner. Sham-operated mice or mice 48 hours after THS were inoculated by oral gavage with C. rodentium and sacrificed approximately 5 hours after the challenge. After that, sample homogenates obtained from the MLNs (b), liver (c), blood (d), and spleen (e) were plated on LB agar culture plates and grown under aerobic condition at 37°C for 12 hours. Subsequently, bacterial CFUs were visualized (a) using IVIS, and bacterial abundance was calculated (b–e). Boxes represent the interquartile range (bottom, 25^th percentile; top, 75^th percentile), and the line inside represents the median. Whiskers denote the lowest and highest values within 1.5x the interquartile range. Each group contained 8 animals. N.S. indicates not significant. All significant values are marked, and is considered significant by one-way ANOVA test. Data are representative of three independent experiments.

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