Granulocyte-Macrophage Colony-Stimulating Factor-Activated Neutrophils Express B7-H4 That Correlates with Gastric Cancer Progression and Poor Patient Survival

Figure 1

Neutrophils are enriched in human GC environment with tumor progression and associated with poor patient survival. (a) Kaplan-Meier plots for intratumoral MPO expression in GC patients (overall survival in Kaplan-Meier plotter website). (b) Expression of MPO between autologous tumor and nontumor tissues () was analyzed. (c) Expression of MPO in GC tumors among TNM stages was compared. Human GAPDH mRNA level served as a normalizer, and its level in nontumor tissues served as a calibrator. The relative gene expression was expressed as fold change of relevant mRNA calculated by the ^ΔΔCt method. (d) Representative analysis of MPO⁺ (brown) neutrophil distributions in tumor and nontumor tissues of GC patients by immunohistochemical staining. Scale bars: 100 microns. MPO: myeloperoxidase. The horizontal bars in (b) and (c) represent mean values. Each ring or dot in (b) and (c) represents 1 patient. , , and ^n.s. for groups connected by horizontal lines.

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