Granulocyte-Macrophage Colony-Stimulating Factor-Activated Neutrophils Express B7-H4 That Correlates with Gastric Cancer Progression and Poor Patient Survival

Figure 3

Human GC environments maintain neutrophil-activated and highly B7-H4-expressing phenotype. (a) Expressions of CD54 and B7-H4 on neutrophils exposed to 50% TTCS and 50% NTCS from autologous GC patients, or to medium control for 12 h. Black: isotype control. MFI: mean fluorescence intensity. (b) Expressions of CD54 and B7-H4 on neutrophils exposed to 50% TTCS from GC patients for 3, 6, or 12 h. Black: isotype control. MFI: mean fluorescence intensity. (c) Expressions of CD54 and B7-H4 on neutrophils exposed to 10%, 20%, or 50% TTCS from GC patients for 12 h. Black: isotype control. MFI: mean fluorescence intensity. and for groups connected by horizontal lines.

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