Granulocyte-Macrophage Colony-Stimulating Factor-Activated Neutrophils Express B7-H4 That Correlates with Gastric Cancer Progression and Poor Patient Survival

Figure 5

Tumor-derived GM-CSF activates neutrophils and induces B7-H4 expression on neutrophils via activating the JAK-STAT3 pathway. (a) Expressions of CD54 and B7-H4 on neutrophils exposed to 50% TTCS with anti-GM-CSF antibody or 50% NTCS with GM-CSF for 12 h. Black: isotype control. (b) Expressions of CD54 and B7-H4 on neutrophils exposed to 50% TTCS pretreated with anti-GM-CSF receptor (GM-CSFR) antibody for 12 h. Black: isotype control. (c) GM-CSF concentration between autologous tumor and nontumor tissues () or between autologous TTCS and NTCS () was analyzed. (d) Expressions of CD54 and B7-H4 on neutrophils exposed to 50% TTCS or GM-CSF with or without JAK signal transduction inhibitor AG490 for 12 h. (e) Expressions of CD54 and B7-H4 on neutrophils exposed to 50% TTCS or GM-CSF with or without STAT3 phosphorylation inhibitor FLLL32 for 12 h. (f) STAT3 and p-STAT3 in neutrophils exposed to 50% TTCS and 50% NTCS from autologous GC patients, or to medium control, or to 50% TTCS with anti-GM-CSF antibody or control IgG were analyzed by western blot. Each dot in (b) represents 1 patient. for groups connected by horizontal lines.

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