Inhibition of NF-κB/IL-33/ST2 Axis Ameliorates Acute Bronchiolitis Induced by Respiratory Syncytial Virus

Figure 2

NF-κB/IL-33/ST2 axis is mediated the Th2 cytokine levels and BAL cell number induced by RSV. (a) Western blot analysis was performed to examine the abundance of p-IκBa, ST2, p-p65, and p-p50. (b, c) RT-PCR analysis was used to examine the mRNA expression of Th2-related cytokines IL-4 and IL-10 after IL-33 antibody treatment. (d) Inflammatory cell number of bronchoalveolar lavage fluid after IL-33 antibody treatment. (e) ST2 protein abundance was examined by western blot analysis. (f) The IL-33 mRNA expression in lung tissues was detected by RT-PCR. (g, h) RT-PCR analysis was used to examine the mRNA expression of Th2-related cytokines IL-4 and IL-10 after PDTC treatment. (i) Inflammatory cell number of bronchoalveolar lavage fluid after PDTC treatment. (j, k) PCR analysis was used to examine the mRNA expression of Th2-related cytokines IL-4 and IL-10 after sST2 treatment. (l) Inflammatory cell number of bronchoalveolar lavage fluid after sST2 treatment. All data were expressed as the (). Each value of , , and was considered to be significant differences.

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