Prolactin Increases the Frequency of Follicular T Helper Cells with Enhanced IL21 Secretion and OX40 Expression in Lupus-Prone MRL/lpr Mice

<div>Differentiation and survival of T<sub>FH</sub> cells in the presence of PRL. T<sub>naïve</sub> cells from 9-week-old C57BL/6 and MRL/lpr mice were purified by MACS and differentiated to T<sub>FH</sub> in the presence and absence of PRL for 48 h, before staining with a viability marker (Ghost Red) and anti-Ki-67, anti-CD4, anti-CXCR5, anti-BCL6, and anti-ICOS antibodies. The surface CXCR5<sup>+</sup>PD1<sup>+</sup> population represents T<sub>FH</sub> cells. (a) Percentage of differentiation to T<sub>FH</sub> in vitro. (b) Zebra plot of one representative experiment. (c) Expression of BCL6, CXCR5, and ICOS (MFI) in T<sub>FH</sub> cells. (d) Percentage of T<sub>FH</sub> cell survival. (e) Percentage of proliferation (Ki-67<sup>+</sup>). Six different experiments were performed; each experiment was done in triplicate. 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Journal of Immunology Research

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Figure 4

Figure 4: Prolactin Increases the Frequency of Follicular T Helper Cells with Enhanced IL21 Secretion and OX40 Expression in Lupus-Prone MRL/lpr Mice 

Figure 4 | Prolactin Increases the Frequency of Follicular T Helper Cells with Enhanced IL21 Secretion and OX40 Expression in Lupus-Prone MRL/lpr Mice 