Tissue-Resident Memory-Like CD8⁺ T Cells Exhibit Heterogeneous Characteristics in Tuberculous Pleural Effusion

Figure 6

Upregulation of CD103 and CD69 on CD8⁺ T cells by TGF-β and IFN-α. PFMCs were stimulated with or without anti-CD28 in the presence or absence of BCG for 48 hours, and then, CD69 and CD103 expression on CD8⁺ T cells were detected; the (a) representative dot plots and (b) statistical data were shown (). PFMCs were stimulated with anti-CD3 plus anti-CD28 (neutral condition) in the presence or absence of TNF-α, TGF-β, IFN-α, and IFN-γ, the frequencies of CD69⁺CD103^-, CD69⁺CD103⁺, and CD69^-CD103⁺ cells were analyzed, and the (c) representative dot plots and (d) statistical data were shown (). PFMCs were stimulated with neutral condition in the presence or absence of different doses of TGF-β, the frequencies of CD69⁺CD103^-, CD69⁺CD103⁺, and CD69^-CD103⁺ cells were analyzed, and the (e) representative dot plots and (f) statistical data were shown (). , , and .

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