Leukocytes from Patients with Drug-Sensitive and Multidrug-Resistant Tuberculosis Exhibit Distinctive Profiles of Chemokine Receptor Expression and Migration Capacity

Figure 2

Increased frequency of CD14+CD3+ monocytes during DS-TB and MDR-TB. Representative zebra plots illustrating the gating strategy used to analyze CD3 and CD16 expressions in total CD14+ cells. Peripheral blood mononuclear cells (PBMCs) from multidrug-resistant tuberculosis (MDR-TB) and drug-sensitive tuberculosis (DS-TB) patients were obtained at different time points and employed for phenotypical characterization by flow cytometry (a and c, respectively). (b) Frequency of CD14+CD3+, CD14+CD3+CD16+, and CD14+CD16+ subpopulations in MDR-TB patients (HD: ; MDR-TB: baseline and 1 and 3 moTBt , 8 moTBt , 16 moTBt ) (d) Frequency of CD14+CD3+, CD14+CD3+CD16+, and CD14+CD16+ subpopulations in DS-TB patients (HD: ; DS-TB: baseline and 1 moTBt , 6 moTBt ). Bar graphs show . Differences between patient groups and HD at each time point were analyzed using multiple Student’s -test corrected for multiple comparisons using the Sidak–Bonferroni method. , , , and . moTBt: months of antituberculosis treatment.

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