High Expression of VSTM2L Induced Resistance to Chemoradiotherapy in Rectal Cancer through Downstream IL-4 Signaling

Figure 4

Overexpression of VSTM2L induced resistance to CRT through downstream IL-4 signaling. (a) The classification of rectal cancer patients and LS.174 cancer cell was divided into high and low expression groups from GSE45404, GSE68204, GSE87211, and VSTM2L-overpressing cell RNA-sequence data according to the mRNA expression level of VSTM2L. . (b, c) Upregulation of VSTM2L expression was positively correlated with IL-4 signaling in rectal cancer as predicted by GSEA (GSEA: gene set enrichment analysis; NES: normalized enrichment , value < 0.001). (d) The heat map showed that the overexpression of VSTM2L was associated with downstream IL-4 signaling enrichment-related 13 candidate genes as the RNA-Seq data analysis (), including 10 upregulated genes and 3 downregulated genes. (e) Overexpression of VSTM2L in LS.174T and SW837 cell affected the protein expression of downstream IL-4 signaling genes which proved the effects of promoting cell proliferation and inhibiting apoptosis.

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