Epitope-Based Chicken-Derived Novel Anti-PAD2 Monoclonal Antibodies Inhibit Citrullination

Figure 4

Ability of anti-PAD2 antibodies to inhibit recombinant human PAD2- (rhPAD2-) mediated histone citrullination. The anti-PAD2 antibodies were mixed with rhPAD2 and buffer as described in the legend of Figure 3 at 25°C for 30 min. Histone (1.8 mg/mL) and CaCl₂ (1 mM) were added simultaneously and the mixture was incubated for 4 h at 37°C. Citrullinated histone was determined using a colorimetric assay.