Epitope-Based Chicken-Derived Novel Anti-PAD2 Monoclonal Antibodies Inhibit Citrullination

Table 2

Alanine scanning of epitopes along with their half-maximal effective concentration. EC₅₀ (ng/mL) values determined using enzyme-linked immunosorbent assay.

Residue no.	341	342	343	344	345	346	347	348	349	350	351	352	353	354	355	356	357	EC50, original epitope
Amino acid	Y	L	N	R	G	D	R	W	I	Q	D	E	I	E	F	G	Y
Anti-PAD2 mAb S4	6.12			42.72			1.68 × 10^7			N.D.			105.90			9.17		4.45
Anti-PAD2 mAb S10	6.18			32.82			659.60			4.25 × 10^5			1.64 × 10^12			9.08		4.49
Anti-PAD2 mAb S24	7.15			123.90			1550.00			119.70			250.70			9.16		5.08
Anti-PAD2 mAb S108	4.93			12.56			50.09			N.D.			1.30 × 10^5			5.76		4.16
Anti-PAD2 mAb S170	3.01			553.30			1.24 × 10^7			6.24 × 10^5			N.D.			4.35		2.15
Anti-PAD2 mAb S309	19.25			295.40			332.60			N.D.			N.D.			31.90		11.53

Bold region showed no significant reactivity in alanine scanning.