Increased Nuclear Transporter KPNA2 Contributes to Tumor Immune Evasion by Enhancing PD-L1 Expression in PDAC

Figure 2

KPNA2 knockdown inhibited the growth of PDAC cells in vivo rather than in vitro. (a, b) KPNA2 silencing by sh-RNA-KPNA2 in MIA PaCa-2 and PANC-1 cells. (c) CCK8 assays of the control group and KPNA2 knockdown group of MIA PaCa-2 and PANC-1 cell lines. (d) Wound healing assays of the control group and KPNA2 knockdown group of MIA PaCa-2 and PANC-1 cell lines. (e) KPNA2 silencing by sh-RNA-KPNA2 in KPC1199-luc cell. (f) Bioluminescence imaging and luminescence intensity of orthotopic tumor growth. (g) Pancreas of C57BL/6N mice orthotopically transplanted stable KPNA2-knockdown KPC1199-luc cells and control cells. (h) Representative images of H&E staining of orthotopic PDAC model mice at 21 days.

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