Increased Nuclear Transporter KPNA2 Contributes to Tumor Immune Evasion by Enhancing PD-L1 Expression in PDAC

Figure 3

Increased KPNA2 promoted PD-L1 expression and thereby contributed to tumor immune evasion. (a) Gene-immune analysis of KPNA2 in PDAC conducted on Sanger box. (b) The correlation between expression levels of KPNA2 and PD-L1 in PDAC. (c) Relative mRNA expression level of PD-L1 target genes in MIA PaCa-2 and PANC-1 cells with sh-KPNA2 or control vector. (d, e) Protein level of PD-L1 on the surface of MIA PaCa-2 and PANC-1 cells with sh-KPNA2 or control vector. (f, g) Relative mRNA expression level and protein level of PD-L1 in KPC1199-luc cells with sh-KPNA2 or control vector. (h) Protein level of PD-L1 on the surface of tumor cells derived from orthotopic PDAC model mice orthotopically transplanted KPC1199-luc cells with sh-KPNA2 or control vector. (i) CD8 infiltration and granzyme B expression in mouse tumor tissue detected by IHC (scale bar: 100 μm).

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