HMSC-Derived Exosome Inhibited Th2 Cell Differentiation via Regulating miR-146a-5p/SERPINB2 Pathway

Figure 4

HMSC-derived exosome inhibited the AR CD4⁺ cells differentiated into Th2 type cells through delivering miR-146a-5p. (a) miR-146a-5p induced silencing and overexpression using corresponding mimic and inhibitor in hMSCs. vs. miNC (b). The relative levels of miR-146a-5p and SERPINB2 in AR CD4⁺ cells after coculture with NC-exo, inhibitor-exo, and mimic-exo. vs. control, vs. control; !!! vs. NC-exo. (c) Western blot analysis was used to quantify the protein contents of SERPINB2, T-bet, and GATA-3 in AR CD4⁺ cells after co-culture with NC-exo, inhibitor-exo, and mimic-exo. (d) The distribution of Th1 and Th2 cells in AR CD4⁺ T cells after co-culture with NC-exo, inhibitor-exo, and mimic-exo. vs. control.

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