circCOL1A1 Promotes the Progression of Gastric Cancer Cells through Sponging miR-145 to Enhance RABL3 Expression

Figure 1

Characterization of circCOL1A1 in gastric cancer. (a) PCR was performed in BGC-823 and AGS. circCOL1A1 was amplified by specially designed divergent primers from cDNA but not from genomic DNA (gDNA). Linear COL1A1 RNA was amplified by convergent primers both in cDNA and gDNA. GAPDH was an endogenous control. (b, c) RNase R was used to pretreat the RNA, and qRT-PCR was used to determine the resistance. circCOL1A1 was resistance to RNase R in BGC-823 and AGS cell lines. (d, e) BGC-823 and AGS were treated with 5 mg/ml actinomycin D for 0, 4, 8, 12, and 16 h. RNAs extracted and determined by qRT-PCR analyses. (f) Expression of circCOL1A1 in different gastric cancer cells and normal cells. (g) qRT-PCR was applied to determine the expression of circCOL1A1 in gastric cancer tissue.

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