circCOL1A1 Promotes the Progression of Gastric Cancer Cells through Sponging miR-145 to Enhance RABL3 Expression

Figure 3

circCOL1A1 has no influence on COL1A1 protein level and mainly locates in the cytoplasm. (a) qRT-PCR was performed to detect COL1A1 linear RNA levels in AGS. (b) qRT-PCR was performed to detect COL1A1 linear RNA level in MKN-45. (c) Western blot was performed to detect COL1A1 protein levels in AGS. (d) Western blot was performed to detect COL1A1 protein levels in MKN-45. (e, f) Subcellular fractionation assay showed that circCOL1A1 was mainly located in the cytoplasm of AGS and MKN-45 cells. U2 and GAPDH were used as nuclear and cytoplasmic controls, respectively.

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