circCOL1A1 Promotes the Progression of Gastric Cancer Cells through Sponging miR-145 to Enhance RABL3 Expression

Figure 4

circCOL1A1 targets miR-145 by acting as a microRNA sponge. (a) Putative binding site between circCOL1A1 and miR-145 is shown. (b, c) circCOL1A1 was silenced in AGS and BGS-823 cells and reexpressed circCOL1A1 in AGS and BGS-823. qRT-PCR was performed. (d, e) circCOL1A1 was overexpressed in MKN-45 and SGC-7901 cells and then silenced circCOL1A1 in circCOL1A1 overexpressing cells. qRT-PCR was performed. (f, g) circCOL1A1 was silenced in AGS and BGS-823 cells and reexpressed circCOL1A1 in AGS and BGS-823. Luciferase reporter assays were performed. (h, i) circCOL1A1 was overexpressed in MKN-45 and SGC-7901 cells and then silenced circCOL1A1 in circCOL1A1 overexpressing cells. Luciferase reporter assays were performed. (j) The wild type and mutation of miR-145 were indicated. (k, l) RNA pull-down assays were performed to validate the direct interaction between miR-145 and circCOL1A1. (m) The expression level of miR-145 in gastric cancer cell lines by qRT-PCR. (n, o) The expression level of miR-145 in gastric cancer tissues by qRT-PCR.

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