circCOL1A1 Promotes the Progression of Gastric Cancer Cells through Sponging miR-145 to Enhance RABL3 Expression

Figure 5

RABL3 is the target of miR-145 and regulated by circCOL1A1. (a) StarBase V3.0 predicted that RABL3 was the target gene of miR-145. The binding site was shown as indicated. (b) Luciferase reporter assays were performed in AGS transfected with wild type and mutant miR-145. (c) Luciferase reporter assays were performed in MKN-45 transfected with wild type and mutant miR-145. (d) Relative expression of RABL3 mRNA in NC and circCOL1A1 silenced AGS cells which were transfected with miR-145 mimics or inhibitors. (e) Relative expression of RABL3 mRNA in Vector and circCOL1A1 overexpressing MKN-45 cells which were transfected with miR-145 inhibitors or mimics. (f) Protein level of RABL3 was identified by western blot in AGS cell line. (g) Protein level of RABL3 was identified by western blot in the MKN-45 cell line. (h, i) RABL3 level was identified in gastric cancer tissue.

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