circCOL1A1 Promotes the Progression of Gastric Cancer Cells through Sponging miR-145 to Enhance RABL3 Expression

Figure 7

circCOL1A/miR-145/RABL3 axis in vivo. (a) AGS/NC and AGS/si-circCOL1A1 cells were subcutaneously injected into the ventral flanks of 5-week-old female nude mice. The tumors were measured every three days when the tumor reached . At the 32th day, all mice were sacrificed, and tumors were excised and photographed. (b) The tumor volumes were calculated according to the formula: . The data indicated mean with SD of 6 tumors. (c) The weight of tumors of six tumors at the 32th day. (d) The expression of miR-145 and RABL3 in tumors of nude mice. (e) AGS was cocultured with macrophages, and flow cytometry assays were carried out to detect the percentage of M1 (CD86+) and M2 (CD206+). (f) MKN-45 was cocultured with macrophages, and flow cytometry assays were carried out to detect the percentage of M1 (CD86+) and M2 (CD206+). (g) The correlation between circCOL1A1 and miR-145. (h) The correlation between miR-145 and RABL3. (i) The correlation between circCOL1A1 and RABL3.

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