MicroRNA-34a Promotes EMT and Liver Fibrosis in Primary Biliary Cholangitis by Regulating TGF-β1/smad Pathway

Figure 4

Cell morphology and the expression of CK19, a-SMA, and collagen I by immune cytochemistry after transfection with miR-34a mimics, inhibitor, and controls. Before immune cytochemistry assay, cell proliferation was inhibited by miR-34a. By immune cytochemistry, we found that miR-34a downregulated CK19 and upregulated α-SMA and collagen I.