MicroRNA-34a Promotes EMT and Liver Fibrosis in Primary Biliary Cholangitis by Regulating TGF-β1/smad Pathway

Figure 5

miR-34a induced EMT and fibrogenesis of HIBEC. (a) Vimentin and FSP-1 mRNA levels (for vimentin, miR-34a mimics vs. NC, ; miR-34a mimics vs. inhibitor, , and miR-34a inhibitor vs. NC, ; for FSP-1, miR-34a mimics vs. NC, ; miR-34a mimic vs. inhibitor, ; and miR-34a inhibitor vs. NC, ). (b) α-SMA and collagen I mRNA levels (for α-SMA, miR-34a mimics vs. NC, ; miR-34a mimics vs. inhibitor, ; and miR-34a inhibitor vs. NC, ; for collagen I, miR-34a mimics vs. NC, ; miR-34a mimics vs. inhibitor, ; and miR-34a inhibitor vs. NC, ). (c) Relative vimentin and FSP-1 protein expressions (for vimentin, miR-34a mimics vs. NC, ; miR-34a mimics vs. inhibitor, ; and miR-34a inhibitor vs. NC, ; for FSP-1, miR-34a mimics vs. NC, ; miR-34a mimics vs. inhibitor,; and miR-34a inhibitor vs. NC, ). (d) Relative α-SMA and collagen I protein expressions (for α-SMA, miR-34a mimics vs. NC, ; miR-34a mimics vs. inhibitor, ; and miR-34a inhibitor vs. NC, ; for collagen I, miR-34a mimics vs. NC, ; miR-34a mimics vs. inhibitor, , and miR-34a inhibitor vs. NC, ).

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