MicroRNA-34a Promotes EMT and Liver Fibrosis in Primary Biliary Cholangitis by Regulating TGF-β1/smad Pathway

Figure 6

miR-34a promoted EMT and fibrosis of HIBEC through the TGF-β1/smad pathway and miR-34 upregulated inflammatory factors. (a) TβR1 and TGF-β1 mRNA levels (for TβR1, miR-34a mimics vs. NC, ; miR-34a mimics vs. inhibitor, ; and miR-34a inhibitor vs. NC, ; for TGF-β1, miR-34a mimics vs. NC, ; miR-34a mimics vs. inhibitor, ; and miR-34a inhibitor vs. NC, ). (b) IL-6 and IL-17 mRNA levels (for IL-6, miR-34a mimics vs. NC, ; miR-34a mimics vs. inhibitor, ; and miR-34a inhibitor vs. NC, ; for IL-17, miR-34a mimics vs. NC, , miR-34a mimics vs. inhibitor, ; and miR-34a inhibitor vs. inhibitor-NC, ). (c) Relative TβR1, TGF-β1, and p-smad2/3 protein expressions (for TβR1, miR-34a mimics vs. NC, ; miR-34a mimics vs. inhibitor, ; and miR-34a inhibitor vs. NC, ; for TGF-β1, miR-34a mimics vs. NC, ; miR-34a mimics vs. inhibitor, ; and miR-34a inhibitor vs. NC, ; for p-smad2/3, miR-34a mimics vs. NC, , ns; miR-34a mimics vs. inhibitor, , and miR-34a inhibitor vs. NC, ). (d) Relative IL-6 and IL-17 protein expressions (for IL-6, miR-34a mimics vs. NC, ; miR-34a mimics vs. inhibitor, ; and miR-34a inhibitor vs. NC, ; for IL-17, miR-34a mimics vs. NC, ; miR-34a mimics vs. inhibitor, ; and miR-34a inhibitor vs. NC, ).

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