ZNF652-Induced circRHOT1 Promotes SMAD5 Expression to Modulate Tumorigenic Properties and Nature Killer Cell-Mediated Toxicity in Bladder Cancer via Targeting miR-3666

Figure 4

miR-3666 is a downstream target of circRHOT1. (a) The identified six putative miRNAs. (b) RNA pull-down assay conducted using circRHOT1 probe, followed by qRT-PCR, to measure the expression level of circRHOT1. (c, d) Putative miRNAs expression in HT-1376 (c) and HTB9 (d) cells measured by RNA pull-down assay using circRHOT1 probe and qRT-PCR. (e) Expression of circRHOT1 in HT-1376 (e) or HTB9 (f) cells determined by RNA pull-down assay using biotin-miR-3666-WT/Mut probe and qRT-PCR. (g) The binding sites between circRHOT1 and miR-3666 that were synthesized. (h, i) Relative luciferase activities of HT-1376 (h) and HTB9 (i) cells cotransfected with miR-3666 mimics/NC mimics and WT/Mut circRHOT1 plasmids. Experiments were conducted in triplicate. and .

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)