Integrated Bioinformatics and Experimental Approaches Identified the Role of NPPA in the Proliferation and the Malignant Behavior of Breast Cancer

Figure 4

NPPA silencing promotes the malignant behavior of breast cancer cells. (a) Heatmap showing the proliferation potential of breast cancer patients in the TCGA database. The top three clusters of patients were identified as the high, medium, and low proliferation groups. (b) Quantification of NPPA mRNA level in the high, medium, and low proliferation breast cancer patients. (c) The proliferation curve of NPPA depleted or control cells. Experiments were performed in 4 different breast cancer cell lines. (d, e) The typical figure (d) and the quantification (e) of MCF-7 cells and MDA-MB-231 cells in the Transwell experiments. Migration (A) and invasion (B) were assessed, respectively, in NPPA depleted or control cells. (f, g) The proliferation curve (f) and the tumor image (g) of MCF-7 nude mouse xenograft. Tumor growth and tumor volume were assessed in NPPA depleted or control MCF-7 cells. Data are represented as , , , and . The data were analyzed using Student’s -test.

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