Integrated Bioinformatics and Experimental Approaches Identified the Role of NPPA in the Proliferation and the Malignant Behavior of Breast Cancer

Figure 5

NPPA was transcriptionally modulated by MZF1 in breast cancer cells. (a) Veen plot showing the mutual transcription factors identified by both PROMO and JASPAR. (b) Dot plot showing the Pearson correlation between mRNA level of NPPA and 11 transcription factors. MZF1 was labeled in red. (c) Dot plot showing the correlation between NPPA and MZF1 mRNA levels in breast cancer patient. (d) The binding motif of MZF1. (e) The binding site of MZF1 to the promoter region of NPPA with PROMO and JASPAR. Binding sites in PROMO and JASPAR were labeled in red and blue, respectively. (f, g) Quantification of MZF1 and NPPA mRNA in MZF1 overexpressed MCF-7 (f) and MDA-MB-231 (g) cells. (h, i) ChIP result showing the binding of MZF1 to the -381~-452 bp of the NPPA promoter region in MZF1 overexpressed MCF-7 (h) and MDA-MB-231 (i) cells. (j, k) Dual luciferase assay showing the relative transcription activity of -300~-500 bp (Firefly/Renilla, RLU) in MZF1 overexpressed MCF-7 (j) and MDA-MB-231 (k) cells. Data are represented as ; , , and . The data were analyzed using Student’s -test.

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