Single Nucleotide Polymorphisms of IL-33 Gene Correlated with Renal Allograft Fibrosis in Kidney Transplant Recipients

Figure 4

rs10975519 mutation on IL-33 gene promoted the mobility and migration of HK2 cells. (a) Wild-type (pCDNA3.1) and mutated (pCMV-IL-33) plasmids of rs10975519 were constructed for further verification. (b) HK2 cells transfected with pCDNA3.1 or pCMV-IL-33(mut) plasmids were wounded by a pipette (c). Quantitation of the migrated cells was measured by the mobility index, which was determined by the formula “.” (d) The HK2 cells transfected with the pCMV-IL-33 or pCDNA3.1 plasmids were seeded in the top chamber for 4 h and 24 h. (e) HK2 cells migrating through the membrane were quantified by the migration index, which is calculated by the formula “” (f) RT-PCR results indicated higher expression of IL-33 mRNA was seen in the mutated group comparing with the pCDNA3.1 group. Abbreviation: RT-qPCR: real-time PCR.

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