Epitope-Based Peptide Vaccine Design against Fructose Bisphosphate Aldolase of Candida glabrata: An Immunoinformatics Approach

Table 5

Promising T-cell epitope (class MHC II alleles) with their position and peptide sequence and IC50 value and rank.

Core sequence	Allele	Start	End	Peptide sequence	IC50	Rank
LFSSHMLDL	HLA-DRB107:01	132	146	KEHGEPLFSSHMLDL	17.8	3.37
	HLA-DPA101	135	149	GEPLFSSHMLDLSEE	93.6	5.05
	HLA-DPB104:01	135	149	GEPLFSSHMLDLSEE	93.6	5.05
	HLA-DPA101:03	133	147	EHGEPLFSSHMLDLS	46	4.82
	HLA-DPB102:01	133	147	EHGEPLFSSHMLDLS	46	4.82
	HLA-DPA102:01	134	148	HGEPLFSSHMLDLSE	59	6.3
	HLA-DPB101:01	134	148	HGEPLFSSHMLDLSE	59	6.3
	HLA-DPA103:01	135	149	GEPLFSSHMLDLSEE	12	1.14
	HLA-DPB104:02	135	149	GEPLFSSHMLDLSEE	12	1.14
IRGSIAAAH	HLA-DRB101:01	81	95	NASIRGSIAAAHYIR	31.5	15.98
	HLA-DRB104:01	81	95	NASIRGSIAAAHYIR	86.5	7.02
	HLA-DRB501:01	81	95	NASIRGSIAAAHYIR	7.3	1.55
	HLA-DQA101:02	80	94	QNASIRGSIAAAHYI	59.3	3.74
	HLA-DQB106:02	80	94	QNASIRGSIAAAHYI	59.3	3.74
	HLA-DQA105:01	81	95	NASIRGSIAAAHYIR	4.6	0.27
	HLA-DQB103:01	81	95	NASIRGSIAAAHYIR	4.6	0.27
YQAGNVVLS	HLA-DRB101:01	227	241	HGVYQAGNVVLSPEI	19.7	11.15
	HLA-DRB109:01	227	241	HGVYQAGNVVLSPEI	80.9	5.58
	HLA-DQA101:02	227	241	HGVYQAGNVVLSPEI	91.3	6.42
	HLA-DQB106:02	227	241	HGVYQAGNVVLSPEI	91.3	6.42
	HLA-DQA105:01	224	238	GNVHGVYQAGNVVLS	7.9	0.96
	HLA-DQB103:01	224	238	GNVHGVYQAGNVVLS	7.9	0.96
VVAALEAAR	HLA-DRB103:01	41	55	SSTVVAALEAARDAK	50.9	2.91
	HLA-DRB109:01	41	55	SSTVVAALEAARDAK	95.1	6.59
	HLA-DRB501:01	41	55	SSTVVAALEAARDAK	15	3.71
	HLA-DQA101:02	40	54	SSSTVVAALEAARDA	38.1	1.93
	HLA-DQB106:02	40	54	SSSTVVAALEAARDA	38.1	1.93
	HLA-DQA105:01	42	56	STVVAALEAARDAKS	16.2	2.87
	HLA-DQB103:01	42	56	STVVAALEAARDAKS	16.2	2.87
IAPAYGIPV	HLA-DRB101:01	94	108	IRSIAPAYGIPVVLH	12.1	6.74
	HLA-DRB107:01	91	105	AHYIRSIAPAYGIPV	34.1	6.37
	HLA-DRB115:01	94	108	IRSIAPAYGIPVVLH	79.2	8.07
	HLA-DQA105:01	94	108	IRSIAPAYGIPVVLH	16.5	2.94
	HLA-DQB103:01	94	108	IRSIAPAYGIPVVLH	16.5	2.94