The Migration of Human Follicular Dendritic Cell-Like Cell Is Facilitated by Matrix Metalloproteinase 3 Expression That Is Mediated through TNFα-ERK1/2-AP1 Signaling

Figure 3

FDCLC migration is induced by TNFα and is dependent on MMP3 expression. FDCLCs were transfected with either the MMP3 siRNA (MMP3i) or a scrambled-RNA-negative control (Scr). The cells were then treated with TNFα, and a migration assay was performed using A Matrigel-coated Transwell system. (a) Disruption of MMP3 expression drastically suppresses TNFα-induced FDCLC migration. The Transwell membranes were stained with hematoxylin and eosin, and the migrated cells that attached to the undersurface of the membrane were counted in 3 different fields under a microscope. Representative images of migrated FDCLCs are shown in (b). MMP3 siRNA significantly reduced MMP3 mRNA expression (c) and MMP3 secretion (d) in both control and TNFα-treated FDCLCs. (e) The length of FDCLC dendrites. MMP3i showed 34% reduction of the length of the dendrites. (f) Representative images of the FDCLC dendrites. Data shown are the mean values with SD of three independent biological replicates; .

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