Downregulation of RIPK4 Expression Inhibits Epithelial-Mesenchymal Transition in Ovarian Cancer through IL-6

Figure 5

RIPK4 affects the IL-6/STAT3 axis in OC, and IL-6 promotes SKOV3 migration and rescues the mobility of RIPK4-silenced cells. (a) RT-PCR results show that the levels of IL-6 target genes and EMT biomarkers are downregulated in RIPK4-silenced OC cell lines compared with control cell lines. The results are shown as the ( per group). (b) ELISA analysis shows that the IL-6 levels in the supernatants of RIPK4-silenced OC cell cultures were significantly lower than those in control culture supernatants (). (c and d) The effects of adding IL-6 (50 μg/ml) to SKOV3-sh-RIPK4-silenced and control OC cells were detected with wound healing assays. IL-6 significantly promoted migration by SKOV3-sh-RIPK4-control cells (). (e and f) Additionally, IL-6 rescued the migratory ability of SKOV3-sh-RIPK4-silenced cells (). The ratio of gap disappearance and the invasive cell number is shown. vs. control (unpaired -test). vs. control (unpaired -test).

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