Gene Regulatory Network of Human GM-CSF-Secreting T Helper Cells

Figure 8

Gene regulatory network of GM-CSF+/GM-CSF− or memory/naïve CD4 T cells. (a, b) Gene regulatory network reflecting the signatures of GM-CSF-positive versus GM-CSF-negative CD4 T cells. (a) A directed network representing binding from source TFs to target peaks assigned to the indicated gene is shown. Source nodes were selected as described (Figure 7, key TFs), and target nodes were selected to be either differentially accessible (as a peak assigned to the respective node gene) and/or to be differentially expressed on the mRNA level in the GM-CSF+/GM-CSF− contrast. To calculate the PageRank [51] as a measure of importance of the TFs (with TFs influencing more genes being more important), the edges were first inverted (not displayed), and the computed PageRank value is represented by the color scale (light to dark green for lower to higher PageRank). (b) Those TFs having a PageRank value higher than the 99-percentile in either the GM-CSF and/or the memory cell network and their corresponding PageRank value in the GM-CSF network are displayed. (c, d) Same as (a, b), but for the memory/naïve CD4 T cell contrast.

(a)

(b)

(c)

(d)