Identification of CTL Epitopes on Efflux Pumps of the ATP-Binding Cassette and the Major Facilitator Superfamily of Mycobacterium tuberculosis

Figure 2

IFN-γ secretion of peptide-induced CTLs was determined with ELISPOT assay. Candidate peptides were used to induce CTLs with peptide-loaded DCs from four healthy PPD⁺ donors in vitro. T cells were stimulated three times at weekly intervals, and rhIL-2 (50 U/mL) and rhIL-7 (10 ng/mL) were added at intervals of 2 days. On day 21, the induced T cells were collected to measure IFN-γ secretion with an ELISPOT assay. (a) Typical chart for each group. (b) Statistical analysis of each peptide in four donors (). Peptide-loaded T2A2 cells or T2A2 cells only were used as the stimulated cells. PBS group was the negative control; PHA was used as the positive control.

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