Identification of CTL Epitopes on Efflux Pumps of the ATP-Binding Cassette and the Major Facilitator Superfamily of Mycobacterium tuberculosis
Table 2
The data of ESI-MS and the binding affinity and stability of peptide/HLA-A0201 complex.
Antigen
Peptide
MFI
FIb
c
Calculated
Observed
Cell
-
-
-
5.64
-
Background
-
-
-
131
-
-
Rv0194
p160
936.25
—a
ND
ND
ND
Rv0933
p186
958.08
959.90
465
2.52
<2 h
p208
987.16
988.57
479
2.59
<2 h
Rv1218c
p24
916.04
916.89
569
3.32
>4 h
P200
989.24
989.78
366
1.75
>4 h
p158
930.02
930.85
439
2.35
>4 h
p280
941.09
939.98
353
1.62
<2 h
p165
987.14
987.69
479
2.6
> 2 h
Rv1819c
p74
926.21
—a
ND
ND
ND
p70
944.25
—a
ND
ND
ND
p122
1025.24
1026.6
177
0.35
ND
Rv2209
p158
872.07
873.4
462
2.54
>4 h
Rv2477c
p182
1051.16
1051.77
379
1.87
>6 h
p316
984.16
984.8
348
1.58
>2 h
p362
991.24
991.85
367
1.72
> 2 h
p265
1116.28
1116.87
315
1.36
ND
p173
1011.27
1011.89
272
1.15
ND
Rv1877
p71
920.12
919.59
419
2.17
>2 h
p95
932.17
—a
ND
ND
ND
p215
960.22
—a
ND
ND
ND
p295
922.16
—a
ND
ND
ND
Rv2994
p223
1019.29
1020.62
430
2.28
<2 h
p100
1047.3
—a
ND
ND
ND
p307
958.27
—a
ND
ND
ND
Rv3728
p264
858.11
—a
ND
ND
ND
p313
1118.38
—a
ND
ND
ND
p110
932.17
—a
ND
ND
ND
p54
1007.15
—a
ND
ND
ND
Rv2459c
p469
904.07
905.42
440
2.36
>4 h
COX-2d
p321
999.6
1000.3
408
2.11
>4 h
aPurification failed. b.c: estimate of the time required for the loss of 50% of the peptide/HLA-A0201 complexes stabilized at time 0 h. dPositive control peptide. ND: not determined. Background group was added with the corresponding solution buffer.
