Knockdown of lncRNA CCAT1 Inhibits the Progression of Colorectal Cancer via hsa-miR-4679 Mediating the Downregulation of GNG10

Figure 3

lncRNA CCAT1 functioned as an hsa-miR-4679 sponge. (a) EdU staining was performed to detect the proliferation ability after upregulation of hsa-miR-4679. (b) The statistical result of (a). (c) WB was performed to detect the levels of proliferation markers. (d) Transwell migration and invasion assays were performed to access the migration and invasion abilities after upregulation of hsa-miR-4679. (e) The statistical results of (d). (f) FACS was performed to analyze the apoptosis rate after upregulation of hsa-miR-4679. (g) The statistical results of (f). (h) WB was performed to detect the levels of proliferation markers. (i) EdU staining was performed to detect the proliferation ability after the treatment with sh-CCAT1, hsa-miR-4679 inhibitors, and sh-CCAT1+hsa-miR-4679 inhibitors. (j) Transwell migration and invasion assays were performed to access the migration and invasion abilities after the treatment with sh-CCAT1, hsa-miR-4679 inhibitors, and sh-CCAT1+hsa-miR-4679 inhibitors. (k) FACS was performed to analyze the apoptosis rate after the treatment with sh-CCAT1, hsa-miR-4679 inhibitors, and sh-CCAT1+hsa-miR-4679 inhibitors. (l) The statistical result of (i). (m) The statistical result of (j). (n) The statistical result of (k). , , and .

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