Knockdown of lncRNA CCAT1 Inhibits the Progression of Colorectal Cancer via hsa-miR-4679 Mediating the Downregulation of GNG10

Figure 4

hsa-miR-4679 regulated the expression of GNG10. (a) Relative expression of AKR1B10, CDA, KLK7, and GNG10 in CRC and normal tissues was quantified using qPCR. (b) WB was performed to detect the level of GNG10 in CRC tissues. (c) The expression level of GNG10 was negatively correlated with the overall survival rate. (d) The size of tumor tissues was significantly increased with GNG10 overexpression. (e) The strategy for the construction of luciferase reporter vectors to detect the binding between hsa-miR-4679 and GNG10. (f) Relative luciferase activity in cotransfection of hsa-miR-4679 and GNG10 group was significantly reduced. (g) Relative expression of GNG10 was regulated by hsa-miR-4679 mimics and hsa-miR-4679 inhibitors. (h) WB was performed to detect the level of GNG10 after the treatment of hsa-miR-4679 mimics and hsa-miR-4679 inhibitors. , , and .

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