HOXA-AS3 Promotes Proliferation and Migration of Hepatocellular Carcinoma Cells via the miR-455-5p/PD-L1 Axis

Figure 4

HOXA-AS3 enhances PD-L1 expression via binding to the 3-UTR of miR-455-5p. (a) Typical images of the subcellular localization of HOXA-AS3 in Hep3B cells as shown by RNA-FISH. (b) Association between HOXA-AS3 expression and PD-L1 expression in HCC cells evaluated by Pearson’s correlation analysis. (c, d) Effects of HOXA-AS3 overexpression or knockdown on PD-L1 expression in HCC cells. (e) Bioinformatics analysis by using starBase. (f) Levels of miR-455-5p and PD-L1 in HCC cells. (f–i) Dual-luciferase reporter assay with binding sites. (j) Effects of miR-455-5p mimics or inhibitor on miR-455-5p expression in HCC cells. (k) Effects of anti-miR-455-5p and HOXA-AS3 shRNA on PD-L1 expression in HCC cells. (l) Overexpression of a fragment of HOXA-AS3 carrying the MRE of miR-455-5p could reverse the inhibition of PD-L1 expression induced by overexpressed miR-455-5p. Data are reported as the of three separate experiments. , , and .

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