Inflamm-Aging-Related Cytokines of IL-17 and IFN-γ Accelerate Osteoclastogenesis and Periodontal Destruction

Figure 1

Effect of IL-17 or/and IFN-γ on differentiation of preosteoclasts. (a–k) In condition 1, BMMs were cultured with M-CSF (30 ng/ml) for 3 days and then with 20 ng/ml RANKL for another 3 days. On day 6, IL-17 or/and IFN-γ of different concentrations was added. (l, m) The results of TRAP staining of each group are presented (). Osteoclasts were recognized as TRAP-positive multinucleated cells (≥3 nuclei). (l) The number of TRAP-positive osteoclasts and (m) the area of TRAP-positive osteoclasts were counted and presented (, ). ^# compared with the control group; ^& compared with the IL-17+IFN-γ group.