Inflamm-Aging-Related Cytokines of IL-17 and IFN-γ Accelerate Osteoclastogenesis and Periodontal Destruction

Figure 4

Effect of IL-17 (5 μg/ml) or/and IFN-γ (1 μg/ml) on proinflammatory cytokine expression in vivo. Immunohistochemical staining was used to examine the level of (a) IL-1β, (b) TNF-α, and (c) IL-6 expression in gingival tissues (). Quantitative analysis results of (d) IL-1β-, (e) TNF-α-, and (f) IL-6-positive cells are shown. The mRNA level of (g) IL-1β, (h) TNF-α, and (i) IL-6 in gingival tissues after 1 week and 2 weeks was detected by RT-qPCR. Data were presented by , . compared with the NC group, ^# compared with the NS group, and ^& compared with the IL-17+IFN-γ group.