Epitope-Based Immunoinformatic Approach on Heat Shock 70 kDa Protein Complex of Cryptococcus neoformans var. grubii

Table 1

List of the fifteen linear conserved surface antigenic epitopes of heat shock 70 kDa protein with their surface accessibility score, antigenicity score, beta-turn score, and hydrophilicity score.

Start	End	Linear peptide^a	Length	Surface score^b	Antigenicity score^c	Beta-turn score^d	Hydrophilicity score^e
79	84	PEVEEY	6	1.748	1.027	0.897	3.317
592	600	ANYVQASEK	9	1.243	1.028	0.964	3.511
593	600	NYVQASEK	8	1.625	1.024	1.002	3.688
594	600	YVQASEK	7	1.335	1.059	0.923	3.214
595	600	VQASEK	6	1.128	1.042	0.887	4.067
468	474	VKSVEKP	7	1.118	1.079	0.959	2.914
705	716	QSEKPKNVNPVI	12	1.3	1.028	1.067	2.875
706	716	SEKPKNVNPVI	11	1.002	1.029	1.075	2.591
707	716	EKPKNVNPVI	10	1.003	1.031	1.039	2.2
504	513	EVEKEEEVTV	10	1.115	1.024	0.717	3.88
735	741	ILNKPKP	7	1.257	1.024	1.097	0.771
735	745	ILNKPKPKPKV	11	1.844	1.043	1.065	1.382
735	746	ILNKPKPKPKVT	12	1.995	1.032	1.057	1.7
469	476	KSVEKPAS	8	1.544	1.031	1.037	4.088
749	756	TPQQPPAQ	8	2.997	1.026	1.14	3.95

Top promising epitopes for their ideal length and physiochemical properties. ^aBepiPred Linear default threshold value 0.249. ^bEmini surface accessibility default threshold value 1.000. ^cKolaskar and Tongaonkar antigenicity default threshold value 1.024. ^dChou and Fasman beta-turn default threshold value 0.950. ^eParker hydrophilicity default threshold value 1.949.