Epitope-Based Immunoinformatic Approach on Heat Shock 70 kDa Protein Complex of Cryptococcus neoformans var. grubii
Table 4
List of the three promising epitope core sequences of heat shock 70 kDa protein that had the highest binding affinity with MHC-II in terms of IC50 and percentile ranks.
Core sequence
Start
End
Allele
Epitope sequence
IC50
Rank
FDYALVQHF
234
248
HLA-DPA101:03
GRDFDYALVQHFAEE
86.1
7.57
234
248
HLA-DPB102:01
GRDFDYALVQHFAEE
86.1
7.57
235
249
HLA-DPA102:01
RDFDYALVQHFAEEF
40.4
4.14
235
249
HLA-/DPB101:01
RDFDYALVQHFAEEF
40.4
4.14
234
248
HLA-DRB101:01
GRDFDYALVQHFAEE
54.3
22.25
233
247
HLA-DRB103:01
GGRDFDYALVQHFAE
28.2
1.65
234
248
HLA-DRB104:05
GRDFDYALVQHFAEE
21.1
1.41
232
246
HLA-DRB107:01
FGGRDFDYALVQHFA
10.7
1.8
235
249
HLA-DRB109:01
RDFDYALVQHFAEEF
81.3
5.61
234
248
HLA-DRB111:01
GRDFDYALVQHFAEE
65.9
10.43
234
248
HLA-DRB501:01
GRDFDYALVQHFAEE
70.8
12.49
FFGGKVLNF
353
367
HLA-DPA101:03
RIQQFFGGKVLNFTL
57.8
5.71
353
367
HLA-DPB102:01
RIQQFFGGKVLNFTL
57.8
5.71
354
368
HLA-DPA102:01
IQQFFGGKVLNFTLN
43.6
4.53
354
368
HLA-DPB101:01
IQQFFGGKVLNFTLN
43.6
4.53
355
369
HLA-DPA103:01
QQFFGGKVLNFTLNQ
72.6
7.73
355
369
HLA-DPB104:02
QQFFGGKVLNFTLNQ
72.6
7.73
352
366
HLA-DQA105:01
ERIQQFFGGKVLNFT
14.4
2.46
352
366
HLA-DQB103:01
ERIQQFFGGKVLNFT
14.4
2.46
353
367
HLA-DRB101:01
RIQQFFGGKVLNFTL
20.4
11.5
353
367
HLA-DRB107:01
RIQQFFGGKVLNFTL
29.2
5.56
FYRQGAFEL
433
447
HLA-DPA101
KVLTFYRQGAFELEA
67.8
3.94
433
447
HLA-DPB104:01
KVLTFYRQGAFELEA
67.8
3.94
433
447
HLA-DPA101:03
KVLTFYRQGAFELEA
63.4
6.11
433
447
HLA-DPB102:01
KVLTFYRQGAFELEA
63.4
6.11
434
448
HLA-DPA102:01
VLTFYRQGAFELEAA
36.5
3.66
434
448
HLA-DPB101:01
VLTFYRQGAFELEAA
36.5
3.66
434
448
HLA-DRB101:01
VLTFYRQGAFELEAA
5.7
1.43
431
445
HLA-DRB107:01
STKVLTFYRQGAFEL
19.1
3.63
434
448
HLA-DRB109:01
VLTFYRQGAFELEAA
51.5
3.35
Top promising epitopes with strong binding affinity and massive global population coverage.
