Tumor Biomarker In-Solution Quantification, Standard Production, and Multiplex Detection

Figure 5

Biomarker detection using flow cytometry. The Luminex strategy detects multiplexed biomarkers using dual wavelengths. The two fluorescent dyes are excited at a wavelength of 635 nm, and the fluorophore is excited at 532 nm. The fluorescent light emitted from the bead is detected at two separate wavelengths, corresponding to a specific biomarker, while the signal produced from each fluorophore is detected at another wavelength.