Toll-Like Receptor Homolog CD180 Expression Is Diminished on Natural Autoantibody-Producing B Cells of Patients with Autoimmune CNS Disorders

Figure 1

Analysis of B cell subsets in neuromyelitis optica spectrum disorder (NMOSD) and multiple sclerosis (MS) patients. (a) Representative flow cytometry plots of four subsets of peripheral blood CD19⁺ B cells defined by CD27 and IgD labeling: CD19⁺CD27^-IgD⁺ naïve, CD19⁺CD27⁺IgD⁺ nonswitched (NS) memory, CD19⁺CD27⁺IgD^- switched (S) memory, and CD19⁺CD27^-IgD^- double-negative (DN) B cells. (b) Flow cytometric analysis of naïve (CD19⁺CD27^-) and memory (CD19⁺CD27⁺) B cells in peripheral blood of NMOSD, MS, and healthy controls (HC). (c) Flow cytometric analysis of the defined four B cell subpopulations in peripheral blood of NMOSD, MS, and healthy controls (HC). Boxes show interquartile ranges (IQR); whiskers indicate the lowest and highest values; horizontal lines represent medians; HC, NMOSD, and MS; .

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