Circular RNA hsa_circ_0000511 Improves Epithelial Mesenchymal Transition of Cervical Cancer by Regulating hsa-mir-296-5p/HMGA1

Figure 3

hsa_circ_0000511 promoted the invasion and migration of SiHa and HeLa cells by regulating hsa-miR-296-5p. Contrary to hsa_circ_0000511, the expression of hsa-miR-296-5p in SiHa and HeLa cells was significantly lower than that in H8 and C-33A cells (a) (; the data were analyzed by one-way analysis of variance, vs. H8, p <0.01; vs. C-33A, ^##p <0.01), and that was increased when hsa_circ_0000511 was silenced (b) (, the data were analyzed by an unpaired two-sided -test, vs. the control, ; vs. si-circ-NC, ^##). The result of dual-luciferase reporter assay (c) shows that hsa-miR-296-5p is a direct target of hsa_circ_0000511 in SiHa (d) and HeLa (e) cells (; the data were analyzed by one-way analysis of variance; vs. circRNA-wt+miRNA NC, ). The proliferation (f, g), invasion (h), and migration (i) of SiHa and HeLa cells were increased because hsa-miR-296-5p was regulated by hsa_circ_0000511; that is to say, the number of cells in a lower Transwell chamber was increased significantly (j, k). In addition, the expression of hsa-miR-296-5p (l) changed when whether hsa_circ_0000511 (m) was silenced or not. (f, g, j–m) ; the data were analyzed by one-way analysis of variance; vs. si-circ+miRNA mimics, , , and ; vs. miRNA mimics, ^#, ^##, and ^###; vs. mimics NC, ^{^}, ^{^^}, and ^{^^^}; vs. si-circ+miRNA inhibitor, ^&& and ^&&&.

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