Circular RNA hsa_circ_0000511 Improves Epithelial Mesenchymal Transition of Cervical Cancer by Regulating hsa-mir-296-5p/HMGA1

Figure 4

HMGA1 is a direct target of hsa-miR-296-5p, and overexpression of HMGA1 can promote proliferation of SiHa and HeLa cells. Like hsa_circ_0000511, HMGA1 was highly expressed in SiHa and HeLa cells than in H8 and C-33A cells (a) (; the data were analyzed by one-way analysis of variance; vs. H8, ; vs. C-33A, ^##). The results of Western blot (b) showed that the protein expressions of HMGA1 were changed when hsa-miR-296-5p was inhibited or not in SiHa (c) and HeLa (d) cells, and a dual-luciferase reporter assay (e) indicated that HMGA1 is a direct target of hsa-miR-296-5p ((c, d) , the data were analyzed by one-way analysis of variance; vs. mimics-NC, and ; vs. si-circ+miRNA inhibitor, ^###; (f, g), vs. HMGA1 3-UTR (wt)+miRNA NC, ). Moreover, CCK8 detection shown that overexpression of HMGA1 could promote proliferation of SiHa (h) and HeLa (i) cells. ; the data were analyzed by one-way analysis of variance; vs. the control, , , and ; vs. si-circRNA, ^###; vs. OV-HMGA1, ^{^^^}; vs. si-circRNA+OV-HMGA1+miRNA mimics, ^&&.

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