Mechanism of Nucleic Acid Sensing in Retinal Pigment Epithelium (RPE): RIG-I Mediates Type I Interferon Response in Human RPE

Figure 7

Type I IFN response alters RPE barrier function. iPS-RPE cells were cultured on transmembrane in 24-well plates, and TER was measured as an indicator of RPE barrier function. Note that treatment with IFN-β at 100 ng/mL (a) or transfection with RNA but not DNA at 0.25 μg/mL (b) could reduce TER, whereas anti-IFN-β could recover the IFN-β-induced (a) and RNA-induced loss of barrier function (c). Normalized TER after 24 h treatment is summarized in (d) as a bar graph indicating changes to the barrier function. Each data point represents biological replicates (). compared with IFN-β- or 3p-hpRNA-induced group with TER loss.

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