Acacetin Protects Myocardial Cells against Hypoxia-Reoxygenation Injury through Activation of Autophagy

<div>Acacetin suppresses apoptosis of H9c2 cells in H/R injury. H9c2 cells were divided into control, H/R model, and H/R + acacetin (12.5, 25, and 50 <i>μ</i>g/ml) groups. (a) Cell apoptosis rates of each group were analyzed by flow cytometry, while cell survival rates were analyzed by DAPI staining. (b–d) Relative protein levels of cleaved Bax and Bcl-2 in each group were measured by Western blot. 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27V0H384Z"></path></g></svg> compared with H/R model group. DAPI: 4<span class="nowrap"><svg height="11.4859pt" id="M16" style="vertical-align:-0.04979992pt" version="1.1" viewbox="-0.0498162 -11.4361 3.89517 11.4859" width="3.89517pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.0091,0,0,-0.0091,0,-5.741)"><path d="M310 541L304 571C290 586 211 619 185 610L80 76L131 52L310 541Z"></path></g></svg>,</span>6-diamidino-2-phenylindole; H/R: hypoxia/reoxygenation.</div>

Journal of Immunology Research

fig3

Figure 3

Figure 3: Acacetin Protects Myocardial Cells against Hypoxia-Reoxygenation Injury through Activation of Autophagy 