Suppression of DLBCL Progression by the E3 Ligase Trim35 Is Mediated by CLOCK Degradation and NK Cell Infiltration

<div>Trim35 is positively associated with NK cell infiltration and inhibits DLBCL progression. (a) Correlation of Trim35 expression with immune cells in DLBCL. (b) Representative IHC images of tumor-infiltrating CD3<sup>+</sup>, CD19<sup>+</sup>, CD16<sup>+</sup>, CD56<sup>+</sup>, and CD68<sup>+</sup> cells from two patients with high and low expression of Trim35. Scale bar, 50 <i>μ</i>m. (c) The difference in CD3/CD19/CD16/CD56/CD68 positivity between the Trim35 subgroups was assessed by unpaired t test analysis. (d, e) Kaplan-Meier OS analysis in DLBCL with respect to phenotypic characteristics. Analysis of an NK cell marker (CD56<sup>+</sup> and CD16<sup>+</sup>) was performed with evaluable immunostaining. <sup>NS</sup><span class="nowrap"><svg height="11.7782pt" id="M31" style="vertical-align:-3.42938pt" version="1.1" viewbox="-0.0498162 -8.34882 44.5261 11.7782" width="44.5261pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M570 304C570 398 525 448 414 448C385 448 343 445 312 434L329 511L321 518C297 504 262 482 244 460L233 411C195 397 159 381 128 358L135 332C160 347 189 360 224 373L111 -147C97 -210 84 -218 17 -231L13 -257L254 -247L259 -218L233 -216C183 -212 177 -202 189 -142L218 -1C238 -10 266 -12 283 -12C351 3 429 48 483 105C543 168 570 242 570 304ZM482 289C482 161 380 33 304 33C278 33 248 51 233 69L303 396C326 400 352 403 369 403C428 403 482 380 482 289Z"></path></g><g transform="matrix(.013,0,0,-0.013,11.342,0)"><path d="M512 -3V55L134 254V256L512 456V514L75 281V230L512 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Journal of Immunology Research

fig2

Figure 2

Figure 2: Suppression of DLBCL Progression by the E3 Ligase Trim35 Is Mediated by CLOCK Degradation and NK Cell Infiltration 