A Novel In Situ Dendritic Cell Vaccine Triggered by Rose Bengal Enhances Adaptive Antitumour Immunity

Figure 1

RB induced cell cycle arrest and cell death. (a) After the treatment for 24 h with different doses of RB, LLCs mainly exhibited G2/M growth arrest. The gating strategy of cell cycle: First, the adherent cells were removed, and the cell cycle was analysed by the cell cycle model version of FlowJo v10. Software programs provided the estimate of cell percentage with fractional DNA content and cell cycle in sub-G1, G1, S, or G2/M phase. Three independent experiments were performed. (b) The percentage of cells with sub-G1 DNA content in LLCs. (c–e) RB induced cell apoptosis and necrosis in a dose- and time-dependent manner. Representative flow cytometry data are presented (c), and three independent experiments were performed (d, e). The gating strategy of cell necrosis: First, the cells were collected, and the voltage was adjusted with the blank control. Then, a new scatterplot was created, the target cells were gated (cross gate), and the scatterplot was finally compensated by FITC and DAPI single-stained controls. The assay was carried out in triplicate and repeated three times. The results are expressed as the . One-way ANOVA was performed, and significance level was defined as , , and .

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