A Novel In Situ Dendritic Cell Vaccine Triggered by Rose Bengal Enhances Adaptive Antitumour Immunity

Figure 2

Cancer cells treated with RB were exposed to calreticulin on the surface (ecto-CRT). (a) Immunofluorescence analysis of ecto-CRT expression on LLC and B16 cells that were treated with RB (10 or 100 μM, respectively) for 24 h. (b, c) LLC exhibited increased ecto-CRT expression in a dose-dependent manner after 30 min of RB treatment. The gating strategy of ecto-CRT expression of LLCs: First, the cells were collected, and the voltage was adjusted to the blank control. Then, a new scatterplot was created, the target cells were gated (cross gate), and the scatterplot was finally compensated by FITC and DAPI single-stained controls. Representative flow cytometry data are presented (b) as the from three representative independent experiments (c). The assay was carried out in triplicate and repeated three times. The results are expressed as the . One-way ANOVA was performed, and the significance level was defined as and .

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