Research Article
Overexpression of TP53INP2 Promotes Apoptosis in Clear Cell Renal Cell Cancer via Caspase-8/TRAF6 Signaling Pathway
Figure 6
Overexpression of TP53INP2 resulted in an inhibition to cellular proliferation in ccRCC cell lines. (a) TP53INP2 overexpression was stably transfected into the 786-O, ACHN, and A498 ccRCC cell lines. The relative mRNA level of TP53INP2 was determined with qRT-PCR. (b) and (c) The level of TP53INP2 protein was determined with western blot. Relative values were estimated with Image J. (d)–(f) CCK-8 assay was made for the detection on the cell viability of 786-O, ACHN, and A498. (g)–(j) The flow cytometry was performed for the detection on the cell viability of 786-O, ACHN, and A498. Data were presented as of three or four independent experiments. ; .
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