miR-214-3p Deficiency Enhances Caspase-1-Dependent Pyroptosis of Microglia in White Matter Injury

<div>miR-214-3p mediates caspase-1 inflammasome activation in microglia. BV-2 microglia cell lines were cultured and transfected with agomir or antagomir of miR-214-3p. (a) FLICA assay staining for caspase-1 (representative dot plot). (b) FLICA assay staining for caspase-1 (statistical data). (c) Immunoblot for procaspase-1 and caspase-1 P20. (d) Relative protein level quantification by normalizing with <i>β</i>-actin. (e) LDH is released by microglia after ex vivo culture for 24 hours. (f) IL-1<i>β</i> release in culture medium. (g) IL-18 release in culture medium. 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Journal of Immunology Research

fig4

Figure 4

Figure 4: miR-214-3p Deficiency Enhances Caspase-1-Dependent Pyroptosis of Microglia in White Matter Injury 